Chemical composition and biological effects of Citrus aurantium var. dulce essential oil

PDF

Authors: Milena D. Vukić, Jovica Branković and Maja B. Ðukić

Volume/Issue: Volume 26: Issue 1

Published online: 23 May 2023

Pages: 14 - 20

DOI: https://doi.org/10.2478/ahr-2023-0003


Abstract

Citrus aurantium var. dulce (sweet orange) belongs to one of the largest genera of the Rutaceae family. The species of thisgenus are consumed worldwide fresh or in form of beverages. They include well-known crops lemons, oranges, mandarins,grapefruits, and limes. Essential oils (EOs) obtained from this species have great economic value since they are mainly producedfrom the peel of the crops, which are considered waste during their industrial processing. Considering, the aim of this study wasto evaluate the chemical composition of the essential oil (EO) obtained from the peel of Citrus aurantium var. dulce, as well as toassess the biological effects by the means of antioxidant and antibacterial activity. Chemical composition analysis performedusing GC and GC/MS analysis revealed that this EO is a rich source of limonene presented in this sample in the amount of93.86% of the total. Obtained results of antioxidant testing indicate better inhibition of ABTS•+ (68.32 ±3.54%) compared to theDPPH• (8.60 ±1.52%). Moreover, the results of the antimicrobial assessment using the disc diffusion method displayed almostno inhibition power of this essential oil towards G- bacteria and yeast strains, while towards G+ bacterial strains weak inhibitionwas observed.


Keywords: essential oil, Citrus, chemical composition, antioxidant activity, antimicrobial activity

PDF

References

Adams, R. P. (2007). Identification of Essential Oil Components By Gas Chromatography/Mass Spectroscopy. Allured Publishing Corporation. Ed., 4.


Akarca, G., & Sevik, R. (2021). Biological Activities of Citrus limon L. and Citrus sinensis L. Peel Essential Oils. Journal of Essential Oil Bearing Plants, 24(6), 1415–1427. https://doi.org/10.1080/0972060X.2021.2022000


Aljaafari, M. N., AlAli, A. O., Baqais, L., Alqubaisy, M., AlAli, M., Molouki, A., Ong-Abdullah, J., Abushelaibi, A., Lai, K.-S., & Lim, S.-H. E. (2021). An Overview of the Potential Therapeutic Applications of Essential Oils. Molecules, 26(3), 628. https://doi.org/10.3390/molecules26030628


Baptista-Silva, S., Borges, S., Ramos, O. L., Pintado, M., & Sarmento, B. (2020). The progress of essential oils as potential therapeutic agents: a review. Journal of Essential Oil Research, 32(4), 279–295. https://doi.org/10.1080/10412905.2020.1746698


Bendahaa, H., Bouchalb, B., el Mounsia, I., Salhic, A., Berrabehd, M., el Bellaouib, M., & Mimounia, M. (2016). Chemical composition, antioxidant, antibacterial and antifungal activities of peel essential oils of Citrus aurantium grown in Eastern Morocco. Der Pharmacia Lettre, 8(4), 239–245.


Bora, H., Kamle, M., Mahato, D. K., Tiwari, P., & Kumar, P. (2020). Citrus Essential Oils (CEOs) and Their Applications in Food: An Overview. Plants, 9(3), 357. https://doi.org/10.3390/plants9030357


Bourgou, S., Rahali, F. Z., Ourghemmi, I., & Saïdani Tounsi, M. (2012). Changes of Peel Essential Oil Composition of Four Tunisian Citrus during Fruit Maturation. The Scientific World Journal, 2012, 1–10. https://doi.org/10.1100/2012/528593


Bousbia, N., Vian, M. A., Ferhat, M. A., Meklati, B. Y., & Chemat, F. (2009). A new process for extraction of essential oil from Citrus peels: Microwave hydrodiffusion and gravity. Journal of Food Engineering, 90(3), 409–413. https://doi.org/10.1016/j.jfoodeng.2008.06.034


Christaki, S., Moschakis, T., Kyriakoudi, A., Biliaderis, C. G., & Mourtzinos, I. (2021). Recent advances in plant essential oils and extracts: Delivery systems and potential uses as preservatives and antioxidants in cheese. Trends in Food Science & Technology, 116, 264–278. https://doi.org/10.1016/j.tifs.2021.07.029


de Araújo, J. S. F., de Souza, E. L., Oliveira, J. R., Gomes, A. C. A., Kotzebue, L. R. V., da Silva Agostini, D. L., de Oliveira, D. L. V., Mazzetto, S. E., da Silva, A. L., & Cavalcanti, M. T. (2020). Microencapsulation of sweet orange essential oil (Citrus aurantium var. dulcis) by liophylization using maltodextrin and maltodextrin/gelatin mixtures: Preparation, characterization, antimicrobial and antioxidant activities. International Journal of Biological Macromolecules, 143, 991–999. https://doi.org/10.1016/j.ijbiomac.2019.09.160


Fisher, K., & Phillips, C. A. (2006). The effect of lemon, orange and bergamot essential oils and their components on the survival of Campylobacter jejuni, Escherichia coli O157, Listeria monocytogenes, Bacillus cereus and Staphylococcus aureus in vitro and in food systems. Journal of Applied Microbiology, 101(6), 1232–1240. https://doi.org/10.1111/j.1365-2672.2006.03035.x


Floegel, A., Kim, D.-O., Chung, S.-J., Koo, S. I., & Chun, O. K. (2011). Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. Journal of Food Composition and Analysis, 24(7), 1043–1048. https://doi.org/10.1016/j.jfca.2011.01.008


Friedman, M., Henika, P. R., & Mandrell, R. E. (2002). Bactericidal Activities of Plant Essential Oils and Some of Their Isolated Constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes, and Salmonella enterica. Journal of Food Protection, 65(10), 1545–1560. https://doi.org/10.4315/0362-028X-65.10.1545


Galovičová, L., Čmiková, N., Vukovic, N., Vukic, M., Kowalczewski, P. Ł., Bakay, L., & Kačániová, M. (2022). Chemical Composition, Antioxidant, Antimicrobial, Antibiofilm and Anti-Insect Activities of Jasminum grandiflorum Essential Oil. Horticulturae, 8(10), 953. https://doi.org/10.3390/horticulturae8100953


Geraci, A., di Stefano, V., di Martino, E., Schillaci, D., & Schicchi, R. (2017). Essential oil components of orange peels and antimicrobial activity. Natural Product Research, 31(6), 653–659. https://doi.org/10.1080/14786419.2016.1219860


González-Mas, M. C., Rambla, J. L., López-Gresa, M. P., Blázquez, M. A., & Granell, A. (2019). Volatile Compounds in Citrus Essential Oils: A Comprehensive Review. Frontiers in Plant Science, 10. https://doi.org/10.3389/fpls.2019.00012


Mahato, N., Sharma, K., Koteswararao, R., Sinha, M., Baral, E., & Cho, M. H. (2019). Citrus essential oils: Extraction, authentication and application in food preservation. Critical Reviews in Food Science and Nutrition, 59(4), 611–625. https://doi.org/10.1080/10408398.2017.1384716


Palazzolo, E., Armando Laudicina, V., & Antonietta Germanà, M. (2013). Current and Potential Use of Citrus Essential Oils. Current Organic Chemistry, 17(24), 3042–3049. https://doi.org/10.2174/13852728113179990122


Popović-Djordjević, J., Cengiz, M., Ozer, M. S., & Sarikurkcu, C. (2019). Calamintha incana: Essential oil composition and biological activity. Industrial Crops and Products, 128, 162–166. https://doi.org/10.1016/j.indcrop.2018.11.003


Proestos, C., Lytoudi, K., Mavromelanidou, O., Zoumpoulakis, P., & Sinanoglou, V. (2013). Antioxidant Capacity of Selected Plant Extracts and Their Essential Oils. Antioxidants, 2(1), 11–22. https://doi.org/10.3390/antiox2010011


Ruiz, B., & Flotats, X. (2014). Citrus essential oils and their influence on the anaerobic digestion process: An overview. Waste Management, 34(11), 2063–2079. https://doi.org/10.1016/j.wasman.2014.06.026


Singh, B., Singh, J. P., Kaur, A., & Yadav, M. P. (2021). Insights into the chemical composition and bioactivities of citrus peel essential oils. Food Research International, 143, 110231. https://doi.org/10.1016/j.foodres.2021.110231


Thielmann, J., & Muranyi, P. (2019). Review on the chemical composition of Litsea cubeba essential oils and the bioactivity of its major constituents citral and limonene. Journal of Essential Oil Research, 31(5), 361–378. https://doi.org/10.1080/10412905.2019.1611671


van den Dool, H., & Dec. Kratz, P. (1963). A generalization of the retention index system including linear temperature programmed gas – liquid partition chromatography. Journal of Chromatography A, 11, 463–471. https://doi.org/10.1016/S0021-9673(01)80947-X


Vieira, A. J., Beserra, F. P., Souza, M. C., Totti, B. M., & Rozza, A. L. (2018). Limonene: Aroma of innovation in health and disease. Chemico-Biological Interactions, 283, 97–106. https://doi.org/10.1016/j.cbi.2018.02.007