Studying of the Antioxidant Capacity of Sweet Wormwood

PDF

Authors: Ingrid Melinda Gyalai, Tímea Süli-Zakar, Csenge Tóth, Marianna Marschall, Tivadar Kiss, Toshpulot Rajabov and Ferenc Lantos

Volume/Issue: Volume 27: Issue 2

Published online: 30 Oct 2024

Pages: 79 - 83

DOI: https://doi.org/10.2478/ahr-2024-0012


Abstract

In the last years, the research on the sweet wormwood (Artemisia annua L.) has increasingly become the focus of oncology science. The bioactive ingredient of the plant is artemisinin, which has been proven to be effective in the treatment of malaria. At the same time, Hungarian and international research groups are also investigating the plant, with the research aimed at the treatment of malignant cancer. In Europe, the therapeutic use of medicinal plants against tumours is realized in relatively few countries, in contrast, phytotherapy research in Asia reports results with a significant therapeutic effect. The aim of our work was to investigate the antioxidant effect of Artemisia annua. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Oxygen radical absorbance capacity (ORAC) laboratory assays proved that the parts of the herb show a significant antioxidant effect, while the seed and the extracted bioactive ingredient artemisinin have no antioxidant capacity at all. On the other hand, aqueous extracts made from leafy shoots showed promising antioxidant capacity values DPPH 10.48 ±0.46. Due to its bitter taste index 1548, it can be used in premixed feed e.g. for piglets.


Keywords: sweet wormwood ( L.), DPPH, ORAC, antioxidant capacity

PDF

References

Balogh, E. (2010). Antioxidáns kapacitás meghatározása és ennek kialakításában szerepet játszó vegyületek vizsgálata bogyós gyümölcsök esetében. PhD értekezés. Budapesti Corvinus Egyetem, Alkalmazott Kémia Tanszék (in Hungarian).


Bilia, A. R., Santomauro, F., Sacco, C., Bergonzi, M. C., & Donato, R. (2014). Essential oil of Artemisia annua L.: An extraordinary component with numerous antimicrobial properties. Complementary and Alternative Medicine. https://doi.org/10.1155/2014/159819


Beibei, G., & Ying, Y. (2017). A comparative review of methods for comparing means using partially paired data. Statistical Methods in Medical Research, 26(3), 1323–1340. https://doi.org/10.1177/0962280215577111


Brisibe, E. A., & Umoren, A. (2009): Nutritional characterisation and antioxidant capacity of different tissues of Artemisia annua L. August 2009. Food Chemistry, 115(4), 1240–1246. https://doi.org/10.1016/j.foodchem.2009.01.033


Cao, G., Alessio, H. M., & Cutler. R. G. (1993). Oxygen-radical absorbancy capacity assay for antioxidants. Free Radical Biology and Medicine, (14), 303–311. https://doi.org/10.1016/0891-5849(93)90027-r


Ćavar, S., Maksimović, M., Vidic, D., & Parić, A. (2012). Chemical Composition and Antioxidant and Antimicrobial Activity of Essential Oil of Artemisia annua L. from Bosnia. Ind. Crop. Prod., 37, 479–485. https://doi.org/10.1016/j.indcrop.2011.07.024


Cornetti, U. (2009). Antioxidant use in nutraceuticals. Clinics in Dermatology, (27), 175–194. https://doi.org/10.1016/j.clindermatol.2008.01.010


Das, S. (2012). Artemisia annua (qinghao): A pharmacological review. International journal of sciences and research, 4573–4577. http://dx.doi.org/10.13040/IJPSR.0975-8232.3(12).4573-77


Dragsted, L. O., Pedersen, A., Hermetter, A., Basu, S., Hansen, M., Haren, G. R., Kall, M., Breinholt, V., Castenmiller, J. J., Stagsted, J., Jakobsen, J., Skibsted, L., Rasmussen, S. E., Loft, S., & Sandström, B. (2004). A gyümölcsök és zöldségek hatása az oxidatív stressz és az antioxidáns védekezés markereire egészséges nemdohányzóknál. A J Clin Nutr, 79(6), 1060–1072.


Efferth, T. (2017). From ancient herb to modern drug: Artemisia annua and artemisinin for cancer therapy. Semin Cancer Biol., (46), 65–83. https://doi.org/10.1016/j.semcancer.2017.02.009


Frankel, E. N., & Meyer, A. S. (2000). The problems of using one-dimensional methods to evaluate multifunctional food and biological antioxidants. Journal of the Science of Food and Agriculture, (80), 1925–1941. https://doi.org/10.1002/1097-0010(200010)80:13<1925::AID-JSFA714>3.0.CO;2-4


Goulden, C. H. (1956). Methods of Statistical Analysis. (2nd ed.) Wiley (pp. 50–55).


Gouveia, S. C., & Castilho, P. C. (2013). Artemisia annua L.: Essential Oil and Acetone Extract Composition and Antioxidant Capacity. Ind. Crop. Prod., 45, 170–181. https://doi.org/10.1016/j.indcrop.2012.12.022


Halliwell, B., & Gutteridge, J. M. C. (2015). Free radicals in Biology and Medicine. (4th ed.). Oxford University Press. https://doi.org/10.1093/acprof:oso/9780198717478.001.0001


Hegedűs, A. (2013). A csonthéjas gyümölcsök antioxidáns hatásában megnyilvánuló genetikai variabilitás jellemzése. Akadémiai Doktori Értekezés. Budapesti Corvinus Egyetem Genetika és Növénynemesítés Tanszék. (in Hungarian).


Hevesi, B. T., Houghton, P. J., Habtemariam, S., & Kéry, A. (2009). Antioxidant and antiinflammatory effect of Epilobium parviflorum Schreb. Phytotherapy Research, 23(5), 719–724. https://doi.org/10.1002/ptr.2725


Ho, W. E., Peh, H. Y., Chan, T. K., & Wong, W. S. F. (2014). Artemisinins: pharmacological actions beyond anti-malarial. Pharmacol. Ther., 142, 126–139. https://doi.org/10.1016/j.pharmthera.2013.12.001


Kim, M. H., Seo, J. Y., Liu, K. H., & Kim, J. S. (2014). Protective Effect of Artemisia annua L. Extract against Galactose-Induced Oxidative Stress in Mice. PLoS ONE, 9. https://doi.org/10.1371/journal.pone.0101486


Munyangi, J., Cornet-Vernet, L., Idumbo, M., Chen, L., Lutgen, P., Perronne, C., Ngombe, N., Bianga, J., Mupenda, B., Lalukala, P., Mergeai, G., Mumba, D., Towler, M., & Weathers, P. (2020). Effect of Artemisia annua and Artemisia afra tea infusions on schistosomiasis in a large clinical trial. Phytomedicine, (78). https://doi.org/10.1016/j.phymed.2018.10.014


Messaili, S., Colas, C., Fougère, L., & Destandau, E. (2020). Combination of molecular network and centrifugal partition chromatography fractionation for targeting and identifying Artemisia annua L. antioxidant compounds. J. Chromatogr. A, 1615. https://doi.org/10.1016/j.chroma.2019.460785


Müller, M. S., Karhagomba, I. B., Hirt, H. M., & Wemakor E. (2000). The potential of Artemisia annua L. as a locally produced remedy for malaria in the tropics: agricultural, chemical and clinical aspects. J. Ethnopharmacol., 73, 487–493. https://doi.org/10.1016/s0378-8741(00)00289-0


Rédei D., Vasas A., & Veres K. (2015). Gyógynövény- és drogismeret gyakorlatok: növényi drogok morfológiai és analitikai vizsgálata. Szegedi Tudományegyetem, 114–119.


Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am. J. Enol. Vitic., 16, 144–158. https://doi.org/10.5344/ajev.1965.16.3.144


Skowyra, M., Gallego, G. M., Segovia, F., & Almajano M. (2014). Antioxidant Properties of Artemisia annua Extracts in Model Food Emulsions. Antioxidants (Basel), 3(1), 116–128. https://doi.org/10.3390/antiox3010116


Steenkamp, V., Gouws, M., Gulumian, M., Elgorashi, E., & van Staden, J. (2006). Studies on antibacterial, anti-inflammatory and antioxidant activity of herbal remedies used in the treatment of benign prostatic hyperplasia and prostatitis. Journal of Ethnopharmacology, 103(1), 71–75. https://doi.org/10.1016/j.jep.2005.07.007


Rédei, D., Vasas, A., & Veres, K. (2015). Gyógynövény – és drogismeret gyakorlatok: növényi drogok morfológiai és analitikai vizsgálata. Szegedi Tudományegyetem, 114–119.


Szent-Györgyi, A. (1928) CLXXIII. Observations on the function of peroxidase systems and the chemistry of the adrenal cortex. Description of a new carbohydrate derivative. Biochem. J., 22, 1387–1409. https://doi.org/10.1042/bj0221387