ISSR Fingerprints and ITS Restriction Cleavage Differs in Selected Native Chilli Pepper Genotypes
ISSR Fingerprints and ITS Restriction Cleavage Differs in Selected Native Chilli Pepper Genotypes
PDFAuthors:
Volume/Issue: Volume 29: Issue 1
Published online: 18 Jun 2026
Pages: 55–60
Abstract
Chilli peppers (Capsicum spp.) belong to very popular and significant horticultural crops, evaluated for their culinary, nutritional, and pharmacological properties. Knowledge about their genetic diversity is important because of their breeding strategies. In this study, restriction profiles of ITS sequences of three different chilli species were analysed for their differences together with ISSR fingerprint profile. A total of eleven genotypes of native chilli peppers from Peru were used in this study. Three different restriction patterns were obtained. Individual species, Capsicum chinesis Jacq, Capsicum frutescens and Capsicum baccatum were distinguished by UPGMA analysis.
Keywords: variability, DNA markers, chilli peppers
References
Alfosea-Simón, M., Simón-Grao, S., Zavala-Gonzalez, E.A., Cámara-Zapata, J.M., Simón, I., Martínez-Nicolás, J.J., Lidón, V., Rodríguez-Ortega, W.M., & García-Sánchez, F. (2020). Application of Biostimulants Containing Amino Acids to Tomatoes Could Favor Sustainable Cultivation: Implications for Tyrosine, Lysine, and Methionine. Sustainability, 12(22), 9729. https://doi.org/10.3390/su12229729
Alfosea-Simón, M., Simón-Grao, S., Zavala-Gonzalez, E.A., Cámara-Zapata, J.M., Simón, I., Martínez-Nicolás, J.J., Lidón, V., & García-Sánchez, F. (2021). Physiological, Nutritional and Metabolomic Responses of Tomato Plants After the Foliar Application of Amino Acids Aspartic Acid, Glutamic Acid and Alanine. Frontiers in Plant Science, 11, 2021. https://doi.org/10.3389/fpls.2020.581234
Baltazar, M., Correia, S., Guinan, K.J., Sujeeth, N., Bragança, R., & Gonçalves, B. (2021). Recent Advances in the Molecular Effects of Biostimulants in Plants: An Overview. Biomolecules, 11(8), 1096. https://doi.org/10.3390/biom11081096
Baqir, H.A., Zeboon, N.H., & Al-Behadili, A.A.J. (2019). The Role and Importance of Amino Acids Within Plants: A Review. Plant Archives, 19(2), 1402–1410.
Betti, L., Canova, A., Maini, P., Merendino, A., & Paolini, M. (1992). Effects of foliar application of an amino-acid-based biostimulant on the response of pepper seedlings to PepMV infection. Advances in Horticultural Science, 6(2), 97–103. https://www.jstor.org/stable/42881634
Botta, A. (2013). Enhancing plant tolerance to temperature stress with amino acids: an approach to their mode of action. Acta Horticulturae, 1009, 29–35. https://doi.org/10.17660/ActaHortic.2013.1009.1
Dziugieł, T., & Wadas, W. (2020). Possibility of increasing early crop potato yield with foliar application of seaweed extracts and humic acids. Journal of Central European Agriculture, 21(2), 300–310. https://doi.org/10.5513/JCEA01/21.2.2576
Ekin, Z. (2011). Some analytical quality characteristics for evaluating the utilization and consumption of potato (Solanum tuberosum L.) tubers. African Journal of Biotechnology, 10(32), 6001–6010.
García-García, A.L., García-Machado, F.J., Borges, A.A., Morales-Sierra, S., Boto, A., & Jiménez-Arias, D. (2020). Pure Organic Active Compounds Against Abiotic Stress: A Biostimulant Overview. Frontiers in Plant Science, 11, 575829. https://doi.org/10.3389/fpls.2020.575829
Gondek, K., & Mierzwa-Hersztek, M. (2021). Effect of Soil Applied L-tryptophan on the Amount of Biomass and Nitrogen and Sulfur Utilization by Maize. Agronomy, 11(12), 2582. https://doi.org/10.3390/agronomy11122582
Gruszecki, R., & Stawiarz, A. (2021). Biostimulants containing amino acids in vegetable crop production. Acta Scientiarum Polonorum Hortorum Cultus, 20(6), 45–57. https://doi.org/10.24326/asphc.2021.6.6
Guo, N., Zhang, S., Gu, M., & Xu, G. (2021). Function, transport, and regulation of amino acids: What is missing in rice. The Crop Journal, 9(3), 530–542. https://doi.org/10.1016/j.cj.2021.04.002
Hayat, S., Hayat, Q., Alyemeni, M.N., Wani, A.S., Pichtel, J., & Ahmad, A. (2012). Role of proline under changing environments: a review. Plant Signaling & Behavior, 7(11), 1456–1466. https://doi.org/10.4161/psb.21949
Hegedűsová, A., Mezeyová, I., & Andrejová, A. (2015). Methods for the determination of selected biologically active substances. Slovak University of Agriculture in Nitra (Metódy stanovenia vybraných biologicky aktívnych látok). In Slovak. Slovak University of Agriculture.
Hill, D., Nelson, D., Hammond, J., & Bell, L. (2021). Morphophysiology of Potato (Solanum tuberosum) in Response to Drought Stress: Paving the Way Forward. Frontiers in Plant Science, 11. https://doi.org/10.3389/fpls.2020.597554
Ierna, A., Parisi, B., & Melilli, M. G. (2022). Overall Quality of “Early” Potato Tubers as Affected by Organic Cultivation. Agronomy, 12(2), 296. https://doi.org/10.3390/agronomy12020296
Kazimierczak, R., Średnicka-Tober, D., Hallmann, E., Kopczyńska, K., & Zarzyńska, K. (2019). The Impact of Organic vs. Conventional Agricultural Practices on Selected Quality Features of Eight Potato Cultivars. Agronomy, 9(12), 799. https://doi.org/10.3390/agronomy9120799
Khan, S., Yu, H., Li, Q., Gao, Y., Sallam, B.N., Wang, H., Liu, P., & Jiang, W. (2019). Exogenous Application of Amino Acids Improves the Growth and Yield of Lettuce by Enhancing Photosynthetic Assimilation and Nutrient Availability. Agronomy, 9(5), 266. https://doi.org/10.3390/agronomy9050266
Kocira, S. (2019). Effect of amino acid biostimulant on the yield and nutraceutical potential of soybean. Chilean Journal of Agricultural Research, 79(1), 17–25. http://dx.doi.org/10.4067/S0718-58392019000100017
Kocira, S., Szparaga, A., Hara, P., Treder, K., Findura, P., Bartoś, P., & Filip, M. (2020). Biochemical and economical effect of application biostimulants containing seaweed extracts and amino acids as an element of agroecological management of bean cultivation. Scientific Reports, 10(1), 17759. https://doi.org/10.1038/s41598-020-74959-0
Kumar, V., Singh, S.P., & Raha, P. (2018). Organic sources use of amino acids based biostimulants and irrigation schedule on yield: Water use efficiency relationship on potato tuber. Journal of Pharmacognosy and Phytochemistry, 7, 1255–1259.
Kuznetsovaa, M.A., Rogozhin, A.N., Smetanina, T.I., Demidova, V.N., & Statsyuk, N.V. (2022). Foliar application of amino acid-based bioorganic stimulator as a tool to increase the yield of potato. AIP Conference Proceedings (pp. 2390). https://doi.org/10.1063/5.0069279
Lombardo, S., Abbate, C., Pandino, G., Parisi, B., Scavo, A., & Mauromicale, G. (2020). Productive and Physiological Response of Organic Potato Grown under Highly Calcareous Soils to Fertilization and Mycorrhization Management. Agronomy, 10(8), 1200. https://doi.org/10.3390/agronomy10081200
Matysiak, K., Kierzek, R., Siatkowski, I., Kowalska, J., Krawczyk, R., & Miziniak, W. (2020). Effect of Exogenous Application of Amino Acids L-Arginine and Glycine on Maize under Temperature Stress. Agronomy, 10(6), 769. https://doi.org/10.3390/agronomy10060769
Noroozlo, Y.A., Souri, M.K., & Delshad, M. (2019a). Stimulation Effects of Foliar Applied Glycine and Glutamine Amino Acids on Lettuce Growth. Open Agriculture, 4(1), 164–172. https://doi.org/10.1515/opag-2019-0016
Noroozlo, Y.A., Souri, M.K., & Delshad, M. (2019b). Effects of Soil Application of Amino Acids, Ammonium, and Nitrate on Nutrient Accumulation and Growth Characteristics of Sweet Basil. Communications in Soil Science and Plant Analysis, 50(22), 2864–2872. https://doi.org/10.1080/00103624.2019.1689249
Perchlik, M., & Tegeder, M. (2018). Leaf Amino Acid Supply Affects Photosynthetic and Plant Nitrogen Use Efficiency under Nitrogen Stress. Plant Physiology, 178(1), 174–188. https://doi.org/10.1104/pp.18.00597
Popko, M., Michalak, I., Wilk, R., Gramza, M., Chojnacka, K., & Górecki, H. (2018). Effect of the New Plant Growth Biostimulants Based on Amino Acids on Yield and Grain Quality of Winter Wheat. Molecules, 23(2), 470. https://doi.org/10.3390/molecules23020470
Radkowski, A., Radkowska, I., Bocianowski, J., Wolski, K., & Bujak, H. (2021a). Effect of Amino Acid and Titanium Foliar Application on Smooth-Stalked Meadow Grass (Poa pratensis L.) Macronutrient Content. Applied Sciences, 11(23), 11421. https://doi.org/10.3390/app112311421
Radkowski, A., Radkowska, I., Bocianowski, J., Cyplik, A., Wolski, K., & Bujak, H. (2021b). Effect of Amino Acids and Effective Microorganisms on Meadow Silage Chemical Composition. Agronomy, 11(6), 1198. https://doi.org/10.3390/agronomy11061198
Rafiee, H., Naghdi Badi, H., Mehrafarin, A., Qaderi, A., Zarinpanjeh, N., Sekara, A., & Zand, E. (2016). Application of Plant Biostimulants as New Approach to Improve the Biological Responses of Medicinal Plants – A Critical Review. Journal of Medicinal Plants, 15(59), 6–39.
Raja, S., Govindakrishnan, P.M., & Chakrabarti, S.K.A. (2020). Review: A Framework for Yield Improvement in Kharif/Rainy Season Potato in the Low Land Tropics. Acta Horticulturae et Regiotecturae, 23(1), 44–55. https://doi.org/10.2478/ahr-2020-0011
Ramadan, M.F. (2016). Potato Lipids. Advances in Potato Chemistry and Technology (2nd ed., pp. 105–116).
Sierras, N., Botta, A., Staasing, L., Martinez, M.J., & Bru, R. (2015). Understanding the effect of amino acids based biostimulant by an enantiomeric analysis of their active principles and a proteomic profiling approach. Acta Horticulturae, 1148, 93–100. DOI:10.17660/ActaHortic.2016.1148.11
Teixeira, W.F., Fagan, E.B., Soares, L.H., Umburanas, R.C., Reichardt, K., & Neto, D.D. (2017). Foliar and Seed Application of Amino Acids Affects the Antioxidant Metabolism of the Soybean Crop. Frontiers in Plant Science, 8. https://doi.org/10.3389/fpls.2017.00327
Teixeira, W.F., Fagan, E.B., Soares, L.H., Soares, J.N., Reichardt, K., & Neto, D.D. (2018). Seed and Foliar Application of Amino Acids Improve Variables of Nitrogen Metabolism and Productivity in Soybean Crop. Frontiers in Plant Science, 9.
Teixeira, W.F., Soares, L.H., Fagan, E.B., Mello, S.C., Reichardt, K., & Dourado-Neto, D. (2020). Amino Acids as Stress Reducers in Soybean Plant Growth Under Different Water-Deficit Conditions. Journal of Plant Growth Regulation, 39, 905–919. https://doi.org/10.1007/s00344-019-10032-z
Teixeira, W.F., Soares, L.H., Reichardt, K., & Neto, D.D. (2021). Application of glycine on soybean plants submitted to water deficit. Australian Journal of Crop Science, 15(10), 1263–1268.
Tsouvaltzis, P., Koukounaras, A., & Siomos, A.S. (2014). Application of amino acids improves lettuce crop uniformity and inhibits nitrate accumulation induced by the supplemental inorganic nitrogen fertilization. International Journal of Agriculture and Biology, 16, 951–955.
Unece Standard FFV–52: Early And Ware Potatoes. United Nations New York and Geneva. (2009). https://unece.org/sites/default/files/datastore/fileadmin/DAM/trade/agr/standard/standard/fresh/FFV-Std/English_052Potatoes.pdf
Wang, G., Xu, M., Wang, W., & Galili, G. (2017). Fortifying Horticultural Crops with Essential Amino Acids: A Review. International Journal of Molecular Sciences, 18(6), 1306. https://doi.org/10.3390/ijms18061306
Wang, D., Deng, X., Wang, B., Zhang, N., Zhu, C., Jiao, Z., Li, R., & Shen, Q. (2019). Effects of foliar application of amino acid liquid fertilizers, with or without Bacillus amyloliquefaciens SQR9, on cowpea yield and leaf microbiota. PLoS ONE, 14(9). https://doi.org/10.1371/journal.pone.0222048
Zamljen, T., Hudina M., Veberič, R., & Slatnar, A. (2021). Biostimulative effect of amino acids and green algae extract on capsaicinoid and other metabolite contents in fruits of Capsicum spp. Chemical and Biological Technologies in Agriculture, 8(1), 63. https://doi.org/10.1186/s40538-021-00260-5