OPTIMISATION OF GROWING CONDITIONS OF EUROPEAN MISTLETOE HYPERPARASITIC FUNGUS (PHAEOBOTRYOSPHAERIA VISCI): EFFECT OF DIFFERENT MEDIA AND ANTIBIOTICS / OPTIMALIZÁCIA RASTOVÝCH PODMIENOK PHAEOBOTRYOSPHAERIA VISCI, HYPERPARAZITICKEJ HUBY IMELA BIELEHO: VPLYV RÔZNYCH ŽIVNÝCH MÉDIÍ A ANTIBIOTÍK
OPTIMISATION OF GROWING CONDITIONS OF EUROPEAN MISTLETOE HYPERPARASITIC FUNGUS (PHAEOBOTRYOSPHAERIA VISCI): EFFECT OF DIFFERENT MEDIA AND ANTIBIOTICS / OPTIMALIZÁCIA RASTOVÝCH PODMIENOK PHAEOBOTRYOSPHAERIA VISCI, HYPERPARAZITICKEJ HUBY IMELA BIELEHO: VPLYV RÔZNYCH ŽIVNÝCH MÉDIÍ A ANTIBIOTÍK
PDFAuthors: Ildikó Varga, Tivadar Baltazár and Miloš Pejchal
Volume/Issue: Volume 16: Issue 2
Published online: 13 Dec 2013
Pages: 44–47
Abstract
European mistletoe (Viscum album) is an evergreen, perennial, hemiparasitic shrub, which is able to infect more than 450 woody species. Besides mechanical pruning, a hyperparasitic fungus (Phaeobotryosphaeria visci) could be a successful candidate to develop an effective biocontrol agent against mistletoe. It was necessary to find antibiotics which make the isolation easier and do not effect mycelia growth negatively under strain. Furthermore, we wanted to define a medium for optimal growth under in vitro laboratory conditions. Three different media (potato-dextrose agar, modified oatmeal agar, ¼ PDA + V8 agar) and four antibiotics (kanamycin, ampicillin, rifampicin, nystatin) were tested. Oatmeal agar and potato-dextrose agar are optimal to maintain the fungal strains, the colony diameter was 9 cm on the 12th day. All tested antibiotics are useful, except nystatin.
Keywords: Botryosphaeriaceae, oatmeal agar, antibiotic sensitivity, biological control, Botryosphaeriaceae, ovsený agar, citlivosť na antibiotiká, biologická kontrola
References
BARNEY C. W. - HAWKSWORTH F. G. et al. 1998. Hosts of Viscumalbum. In: Eur. J. For. Path. vol. 28 1998 pp. 187-208.
FARIA J. C. 2011. Resources of Tinn-R GUI/Editor for R Environment. UESC Ilheus Brasil. 2011.
GHANNOUM M. A. - RICE L. B. 1999. Antifungal agents: mode of action mechanisms of resistance and correlation of these mechanisms with bacterial resistance. In: Clin. Microbiol. Rev. vol. 12 1999 pp. 501-517.
HAWKSWORTH F. G. Mistletoes as forest parasites. In: Calder M. - Bernhardt P. (eds) The biology of mistletoes. Acad. P. Sydney Australia. 1983 pp. 317-333. ISBN 0-12-155055-9.
KARADŽIĆ D. - LAZAREV V. Najznačajnije parazitske i saprofitske gljive na beloj imeli (Viscum album L.) i mogućnost njihovog korišćenja u biokontroli. In: Bulletin Faculty of Forestry University of Bajna Luka. 2005 no. 3 pp. 35-46.
KOHANSKI M. A. - DWYER D. J. - COLLINS J. J. 2010. How antibiotics kill bacteria: from targets to networks. In: Nat. Rev. Microbiol. vol. 8 2010 pp. 423-435.
PHILLIPS A. J. L. - ALVES A. et al. 2008. Resolving the phylogenetic and taxonomic status of dark-spored teleomorph genera in the Botryosphaeriaceae. In: Persoonia vol. 21 2008 pp. 29-55.
PINHEIRO J. - BATES D. - DEBROY S. - SARKAR D. and R Development Core Team (2011) nlme: Linear and Nonlinear Mixed Effects Models. R package version 3.1-100. R Development Core Team (2012) R: A language and environment for statistical computing. R Foundation for Statistical Computing Vienna Austria. ISBN 3-900051-07-0. http://www.R-project.org/
STOJANOVIĆ S. 1989. The investigation of Sphaeropsis visci (Salm.) Sacc. and Colletotrichum gloeosporoides (Sacc.) Penz. parasite on European mistletoe (Viscum album ssp. typicum Beck). In Zastita Bilja. vol. 40 1989 pp. 493-503.
VARGA I. - TALLER J. et al. 2012. Leaf-spot disease on European mistletoe (Viscum album) caused by Phaeobotryosphaeria visci: a potential candidate for biological control. In: Biotech. Lett. vol. 34 2012 no. 6 pp. 1059-1065.