GC, GC/MS analysis, and biological effects of Citrus aurantium amara essential oil


Authors: Milena D. Vukić, Jovica Branković and Marija S. Ristić

Volume/Issue: Volume 26: Issue 1

Published online: 23 May 2023

Pages: 21 - 27

DOI: https://doi.org/10.2478/ahr-2023-0004


Citrus aurantium amara (sour orange) belongs to one of the largest genera of the Rutaceae family. The species of this genus areconsumed worldwide as fresh or in form of beverages. They include well-known crops lemons, oranges, mandarins, grapefruits,and limes. The industrial processing of these fruits produces high amounts of waste (around 50%) which is a valuable sourceof essential oils. Since they are produced mainly from peel, considered waste, these essential oils have great economic value.In that regard, the aim of this study was to evaluate the chemical composition of the essential oil obtained from the peel ofCitrus aurantium amara, as well as to assess the biological effects by the means of antioxidant and antibacterial activity. Resultsof GC and GC/MS analysis characterized this EO as a valuable source of limonene found in the amount of 90.4% of the total.Results of antioxidant activity indicate better inhibition of ABTS•+ (44.93 ±1.45%) compared to the DPPH• (11.03 ±1.08%). Moreover,the results of the antimicrobial assessment using the disc diffusion method displayed low inhibition potency of this essential oiltowards G+ and G- bacteria and yeast strains.

Keywords: essential oil, sour orange, chemical composition, antioxidant activity, antimicrobial activity



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