Biochar with N-Fertilizer Effects on Soil CO2 Emissions and Soil Physical Properties
Biochar with N-Fertilizer Effects on Soil CO2 Emissions and Soil Physical Properties
PDFAuthors: Melinda Molnárová and Ján Horák
Volume/Issue: Volume 28: Issue 2
Published online: 18 Nov 2025
Pages: 111 - 120
Abstract
Biochar has gained attention as a soil amendment due to its potential to mitigate climate change by improving soil properties and reducing greenhouse gas emissions. This study investigates the effects of biochar application and reapplication, in combination with different nitrogen (N) fertilization levels, on soil CO2 emissions and soil physical properties. The field experiment was conducted in a temperate climate zone over a five-year period, with biochar applied at doses of 0, 10, and 20 t.ha−1, and N-fertilizer applied at 0, 108, and 162 kg.N.ha−1. Soil temperature, soil water content (SWC), and CO2 fluxes were monitored biweekly during the 2019 growing season (April–October). Results showed that biochar reapplication significantly reduced cumulative CO2 emissions, particularly at higher application rates and in fertilized treatments. In contrast, a single biochar application led to increased CO2 emissions in some cases. A strong correlation was found between CO2 emissions and soil temperature (p <0.001), while the relationship between CO2 emissions and SWC was not significant (p >0.05) except in one fertilized treatment. These findings suggest that biochar application, particularly when reapplied, can play a role in reducing soil CO2 emissions while influencing soil physical properties. However, further research is needed to assess its long-term effects across various soil types and climatic conditions.
Keywords: biochar, N-fertilization, soil CO2 emissions, soil water content
References
Bond-Lamberty, B., & Thomson, A. (2010). A global database of soil respiration data. Biogeosciences, 7(6), 1915–1926. https://doi.org/10.5194/bg-7-1915-2010
Bond-Lamberty, B., & Thomson, A. (2010). A global database of soil respiration data. Biogeosciences, 7(6), 1915–1926. https://doi.org/10.5194/bg-7-1915-2010
Bovsun, M. A., Castaldi, S., Nesterova, O. V., Semal, Viktoriia. A., Sakara, N. A., Brikmans, A. V., Khokhlova, A. I., & Karpenko, T. Y. (2021). Effect of biochar on soil CO2 fluxes from agricultural field experiments in russian far east. Agronomy, 11(8), 1559. https://doi.org/10.3390/agronomy11081559
Cox, P. M., Betts, R. A., Jones, C. D., Spall, S. A., & Totterdell, I. J. (2000). Acceleration of global warming due to carbon-cycle feedbacks in a coupled climate model. Nature, 408(6809), 184–187. https://doi.org/10.1038/35041539
Crutzen, P. J. (2006). Albedo enhancement by stratospheric sulfur injections: A contribution to resolve a policy dilemma? Climatic Change, 77(3–4), 211. https://doi.org/10.1007/s11354-006-9101-y
Davidson, E. A., & Janssens, I. A. (2006). Temperature sensitivity of soil carbon decomposition and feedbacks to climate change. Nature, 440(7081), 165–173. https://doi.org/10.1038/nature04514
Edenhofer, O. (Ed.). (2015). Climate change 2014: mitigation of climate change. Cambridge University Press.
Elder, J. W., & Lal, R. (2008). Tillage effects on gaseous emissions from an intensively farmed organic soil in North Central Ohio. Soil and Tillage Research, 98(1), 45–55. https://doi.org/10.1016/j.still.2007.10.003
European Commission. (2010). Joint Research Centre. Institute for Environment and Sustainability. Biochar application to soils: A critical scientific review of effects on soil properties, processes and functions. Publications Office. https://data.europa.eu/doi/10.2788/472
Fang, C., Smith, P., Moncrieff, J. B., & Smith, J. U. (2005). Similar response of labile and resistant soil organic matter pools to changes in temperature. Nature, 433(7021), 57–59. https://doi.org/10.1038/nature03138
FAO. (2020). The Contribution of Agriculture to Greenhouse Gas Emissions. http://www.fao.org/economic/ess/environment/data/emission-shares/en/
FAO. (2016). Greenhouse Gas Emissions from Agriculture, Forestry and Other Land Use. http://www.fao.org/3/a-i6340e.pdf
Feng, W., Yang, F., Cen, R., Liu, J., Qu, Z., Miao, Q., & Chen, H. (2021). Effects of straw biochar application on soil temperature, available nitrogen and growth of corn. Journal of Environmental Management, 277, 111331. https://doi.org/10.1016/j.jenvman.2020.111331
Fierer, N., Colman, B. P., Schimel, J. P., & Jackson, R. B. (2006). Predicting the temperature dependence of microbial respiration in soil: A continental-scale analysis. Global Biogeochemical Cycles, 20(3), 2005GB002644. https://doi.org/10.1029/2005GB002644
Gokmenoglu, K. K., & Taspinar, N. (2018). Testing the agriculture-induced EKC hypothesis: The case of Pakistan. Environmental Science and Pollution Research, 25(23), 22829–22841. https://doi.org/10.1007/s11356-018-2330-6
He, X., Du, Z., Wang, Y., Lu, N., & Zhang, Q. (2016). Sensitivity of soil respiration to soil temperature decreased under deep biochar amended soils in temperate croplands. Applied Soil Ecology, 108, 204–210. https://doi.org/10.1016/j.apsoil.2016.08.018
He, Y., Zhou, X., Jiang, L., Li, M., Du, Z., Zhou, G., Shao, J., Wang, X., Xu, Z., Hosseini Bai, S., Wallace, H., & Xu, C. (2017). Effects of biochar application on soil greenhouse gas fluxes: A meta-analysis. GCB Bioenergy, 9(4), 743–755. https://doi.org/10.1111/gcbb.12376
Heimann, M., & Reichstein, M. (2008). Terrestrial ecosystem carbon dynamics and climate feedbacks. Nature, 451(7176), 289–292. https://doi.org/10.1038/nature06591
Huang, C., Chen, Y., Jin, L., & Yang, B. (2024). Properties of biochars derived from different straw at 500 °C pyrolytic temperature: Implications for their use to improving acidic soil water retention. Agricultural Water Management, 301, 108953. https://doi.org/10.1016/j.agwat.2024.108953
IPPC (Intergovernmental panel on climate change) (Ed.). (2014). Climate change 2013: The physical science basis. Cambridge university press.
Ippolito, J. A., Laird, D. A., & Busscher, W. J. (2012). Environmental benefits of biochar. Journal of Environmental Quality, 41(4), 967–972. https://doi.org/10.2134/jeq2012.0151
Jeffry, L., Ong, M. Y., Nomanbhay, S., Mofijur, M., Mubashir, M., & Show, P. L. (2021). Greenhouse gases utilization: A review. Fuel, 301, 121017. https://doi.org/10.1016/j.fuel.2021.121017
Jin, J., Li, Y., Zhang, J., Wu, S., Cao, Y., Liang, P., Zhang, J., Wong, M. H., Wang, M., Shan, S., & Christie, P. (2016). Influence of pyrolysis temperature on properties and environmental safety of heavy metals in biochars derived from municipal sewage sludge. Journal of Hazardous Materials, 320, 417–426. https://doi.org/10.1016/j.jhazmat.2016.08.050
Knorr, W., Prentice, I. C., House, J. I., & Holland, E. A. (2005). Long-term sensitivity of soil carbon turnover to warming. Nature, 433(7023), 298–301. https://doi.org/10.1038/nature03226
Kotuš, T., Šimanský, V., Drgoňová, K., Illéš, M., Wójcik-Gront, E., Balashov, E., Buchkina, N., Aydın, E., & Horák, J. (2022). Combination of biochar with n-fertilizer affects properties of soil and n2o emissions in maize crop. Agronomy, 12(6), 1314. https://doi.org/10.3390/agronomy12061314
Krull, E. S., Skjemstad, J. O., & Baldock, J. A. (2004). Functions of Soil Organic Matter and the Effect on Soil Properties: GRDC Project No CSO 00029, Residue Management, Soil Organic Carbon and Crop Performance. CSIRO Land & Water.
Li, L., Awada, T., Shi, Y., Jin, V. L., & Kaiser, M. (2025). Global greenhouse gas emissions from agriculture: Pathways to sustainable reductions. Global Change Biology, 31(1), e70015. https://doi.org/10.1111/gcb.70015
Mukherjee, A., Lal, R., & Zimmerman, A. R. (2014). Effects of biochar and other amendments on the physical properties and greenhouse gas emissions of an artificially degraded soil. Science of The Total Environment, 487, 26–36. https://doi.org/10.1016/j.scitotenv.2014.03.141
Peters, G. P., Andrew, R. M., Boden, T., Canadell, J. G., Ciais, P., Le Quéré, C., Marland, G., Raupach, M. R., & Wilson, C. (2013). The challenge to keep global warming below 2 °C. Nature Climate Change, 3(1), 4–6. https://doi.org/10.1038/nclimate1783
Petersen, H., & Luxton, M. (1982). A comparative analysis of soil fauna populations and their role in decomposition processes. Oikos, 39(3), 288. https://doi.org/10.2307/3544689
Post, W. M., Emanuel, W. R., Zinke, P. J., & Stangenberger, A. G. (1982). Soil carbon pools and world life zones. Nature, 298(5870), 156–159. https://doi.org/10.1038/298156a0
Raich, J. W., & Tufekciogul, A. (2000). Vegetation and soil respiration: Correlations and controls. Biogeochemistry, 48(1), 71–90. https://doi.org/10.1023/A:1006112000616
Razzaghi, F., Obour, P. B., & Arthur, E. (2020). Does biochar improve soil water retention? A systematic review and meta-analysis. Geoderma, 361, 114055. https://doi.org/10.1016/j.geoderma.2019.114055
Ridzuan, N. H. A. M., Marwan, N. F., Khalid, N., Ali, M. H., & Tseng, M.-L. (2020). Effects of agriculture, renewable energy, and economic growth on carbon dioxide emissions: Evidence of the environmental Kuznets curve. Resources, Conservation and Recycling, 160, 104879. https://doi.org/10.1016/j.resconrec.2020.104879
Romaní, A. M., Fischer, H., Mille-Lindblom, C., & Tranvik, L. J. (2006). Interactions of bacteria and fungi on decomposing litter: Differential extracellular enzyme activities. Ecology, 87(10), 2559–2569. https://doi.org/10.1890/0012-9658(2006)87[2559:IOBAFO]2.0.CO;2
Rothenberg, G. (2023). A realistic look at CO2 emissions, climate change and the role of sustainable chemistry. Sustainable Chemistry for Climate Action, 2, 100012. https://doi.org/10.1016/j.scca.2023.100012
Scharlemann, J. P., Tanner, E. V., Hiederer, R., & Kapos, V. (2014). Global soil carbon: Understanding and managing the largest terrestrial carbon pool. Carbon Management, 5(1), 81–91. https://doi.org/10.4155/cmt.13.77
Shackley, S., Ruysschaert, G., Zwart, K., & Glaser, B. (Ed.). (2016). Biochar in European soils and agriculture: Science and practice. Routledge.
Shafawi, A. N., Mohamed, A. R., Lahijani, P., & Mohammadi, M. (2021). Recent advances in developing engineered biochar for CO2 capture: An insight into the biochar modification approaches. Journal of Environmental Chemical Engineering, 9(6), 106869. https://doi.org/10.1016/j.jece.2021.106869
Tang, J., Baldocchi, D. D., Goldstein, A., & Xu, L. (2003). Pulse effects of soil respiration after rain events in California. https://ui.adsabs.harvard.edu/abs/2003AGUFM.B52D..05T
Tang, S., Cheng, W., Hu, R., Guigue, J., Kimani, S. M., Tawaraya, K., & Xu, X. (2016). Simulating the effects of soil temperature and moisture in the off-rice season on rice straw decomposition and subsequent CH4 production during the growth season in a paddy soil. Biology and Fertility of Soils, 52(5), 739–748. https://doi.org/10.1007/s00374-016-1114-8
Tisserant, A., & Cherubini, F. (2019). Potentials, limitations, co-benefits, and trade-offs of biochar applications to soils for climate change mitigation. Land, 8(12), 179. https://doi.org/10.3390/land8120179
Vargas, R., Detto, M., Baldocchi, D. D., & Allen, M. F. (2010). Multiscale analysis of temporal variability of soil CO2 production as influenced by weather and vegetation. Global Change Biology, 16(5), 1589–1605. https://doi.org/10.1111/j.1365-2486.2009.02111.x
Wang, J., & Wang, S. (2019). Preparation, modification and environmental application of biochar: A review. Journal of Cleaner Production, 227, 1002–1022. https://doi.org/10.1016/j.jclepro.2019.04.282
Weber, K., & Quicker, P. (2018). Properties of biochar. Fuel, 217, 240–261. https://doi.org/10.1016/j.fuel.2017.12.054
Werner, C., & Brantley, S. (2003). CO2 emissions from the Yellowstone volcanic system. Geochemistry, Geophysics, Geosystems, 4(7), 2002GC000473. https://doi.org/10.1029/2002GC000473
Xiong, J., Yu, R., Islam, E., Zhu, F., Zha, J., & Sohail, M. I. (2020). Effect of biochar on soil temperature under high soil surface temperature in coal mined arid and semiarid regions. Sustainability, 12(19), 8238. https://doi.org/10.3390/su12198238
Xu, L., Madsen, R., Anderson, D., Furtaw, M., Garcia, R., & McDermitt, D. (2004). The impact of wind on the soil respiration measurement. American Geophysical Union, Fall Meeting, B51A-0935. https://ui.adsabs.harvard.edu/abs/2004AGUFM.B51A0935X
Xu, M., & Shang, H. (2016). Contribution of soil respiration to the global carbon equation. Journal of Plant Physiology, 203, 16–28. https://doi.org/10.1016/j.jplph.2016.08.007
Yoo, G., & Kang, H. (2012). Effects of biochar addition on greenhouse gas emissions and microbial responses in a short-term laboratory experiment. Journal of Environmental Quality, 41(4), 1193–1202. https://doi.org/10.2134/jeq2011.0157
Zhang, Q., Lei, H.-M., & Yang, D.-W. (2013). Seasonal variations in soil respiration, heterotrophic respiration and autotrophic respiration of a wheat and maize rotation cropland in the North China Plain. Agricultural and Forest Meteorology, 180, 34–43. https://doi.org/10.1016/j.agrformet.2013.04.028
Zhang, G., Yu, H., Fan, X., Liu, G., Ma, J., & Xu, H. (2015). Effect of rice straw application on stable carbon isotopes, methanogenic pathway, and fraction of CH4 oxidized in a continuously flooded rice field in winter season. Soil Biology and Biochemistry, 84, 75–82. https://doi.org/10.1016/j.soilbio.2015.02.008
Zhang, C., Zeng, G., Huang, D., Lai, C., Chen, M., Cheng, M., Tang, W., Tang, L., Dong, H., Huang, B., Tan, X., & Wang, R. (2019). Biochar for environmental management: Mitigating greenhouse gas emissions, contaminant treatment, and potential negative impacts. Chemical Engineering Journal, 373, 902–922. https://doi.org/10.1016/j.cej.2019.05.139
Zhou, W., Hui, D., & Shen, W. (2014). Effects of soil moisture on the temperature sensitivity of soil heterotrophic respiration: A laboratory incubation study. PLoS ONE, 9(3), e92531. https://doi.org/10.1371/journal.pone.0092531
Zhu, X., Zhu, T., Pumpanen, J., Palviainen, M., Zhou, X., Kulmala, L., Bruckman, V. J., Köster, E., Köster, K., Aaltonen, H., Makita, N., Wang, Y., & Berninger, F. (2020). Short-term effects of biochar on soil CO2 efflux in boreal Scots pine forests. Annals of Forest Science, 77(2), 59. https://doi.org/10.1007/s13595-020-00960-2